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Dolomite Drop-seq

The Dolomite Single-Cell RNA-Seq System is now available at MHTP Medical Genomics Facility to enable transcriptome profiling of individual cells

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  • Determine transcriptional similarities and differences within a cell population
  • Identify rare cellular subsets
  • Trace lineage and developmental relationships
  • Characterise gene co-expression patterns

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  • Multiple flow control pumps to regulate pressure and droplet size
  • Re-usable microfluidic chips for cost-effective use
  • High-speed camera and microscope for monitoring droplet production in real-time
  • Versatile and adaptive system with applications limited only by imagination

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scRNA-Seq System Benefits:

  • Fast: mRNA for ~6,000 single-cell libraries captured in a 15-minute run
  • Reproducible: Microfluidic droplet technology enables encapsulation of single cells
  • Flexible: Second channel allows co-encapsulation of cells with oligonucleotide-barcoded beads and lysis buffer

Why droplets?

  • Droplets can be used as micro-compartments or micro-reactors
  • Single cells (or single nuclei) are captured in individual droplets
  • This allows thousands of reproducible compartmentalised reactions to be carried out on single cells in parallel (e.g. RT-PCR)

Individual cells are captured in droplets with mRNA-capture beads

  • Each bead has a unique 12bp DNA sequence ‘barcode’
  • Barcode is followed by a 8bp UMI
  • There are 16 million unique barcodes in the bead library

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The cells are lysed, and the released mRNA is captured on the beads. Emulsion is broken, recovering beads with coupled mRNA for sequencing library generation


  • Please email the Facility This email address is being protected from spambots. You need JavaScript enabled to view it. requesting an initial consultation for your Dolomite project
  • Upon quotation acceptance, please submit your samples according to our sample submission guidelines