For RNAseq gene expression studies investigating differential expression, we recommend a minimum of 30 million reads per sample using 50-100 bp fragment reads.
TruSeq® Stranded mRNA LT - poly-A enrichment
For RNAseq requiring the investigation of coding, non-coding regulation and alternative splice variants, 50-60 million reads per sample is recommended using 100 bp paired-end reads.
TruSeq® Stranded Total RNA LT - (with Ribo-‐Zero Human/Mouse/Rat) - rRNA depletion
Ovation® RNA-Seq System V2 - SPIA technology
Total RNA that has been DNase treated (on column) needs to be supplied with a RIN score > 8 as determined by Agilent Bioanalyser analysis.