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Gene Expression qPCR Resources

The facility hosts two Applied Biosystems Quantstudio™ 6 -384 block instruments.
The instruments support SYBR®, Taqman®, standard and fast chemistry and allow 5 colour multiplexing.                    



  • Gene/miRNA Expression                 
  • Genotyping                                               
  • Copy Number Variation              
  • Protein Thermal Shift                           
  • High-Resolution Melt                   
  • Pathogen Detection  


QuantStudio 6 manual

DataAssist™ Software
Real-Time PCR Troubleshooting Tool

Real-Time PCR Systems Chemistry Guide (pdf)
Sigma qPCR Technical Guide (pdf)

Relative Quantitation Using Comparative Ct Getting Started Guide (pdf)
Absolute Quantitation Using Standard Curve Getting Started Guide (pdf)
Allelic Discrimination Getting Started Guide (pdf)

Understanding Curves